Anticorps Monoclonal anti-Ki-67

Ki-67 Monoclonal Antibody for IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B9H2

N° de cat : 66555-6-PBS

Synonymes

KI67, MKI67, 1B9H2, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67

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Galerie de données de validation

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  IHC staining of human tonsillitis using 66555-6-Ig (same clone as 66555-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66555-6-Ig (Ki-67 antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66555-6-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66555-6-Ig (same clone as 66555-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66555-6-Ig (Ki-67 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66555-6-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 66555-6-Ig (same clone as 66555-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66555-6-Ig (Ki-67 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66555-6-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 66555-6-Ig (same clone as 66555-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66555-6-Ig (Ki-67 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66555-6-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 66555-6-Ig (same clone as 66555-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 66555-6-Ig (Ki-67 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66555-6-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 66555-6-Ig (same clone as 66555-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 66555-6-Ig (Ki-67 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66555-6-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 66555-6-Ig (same clone as 66555-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Ki-67 antibody (66555-6-Ig, Clone: 1B9H2 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66555-6-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66555-6-Ig (same clone as 66555-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Ki-67 antibody (66555-6-Ig, Clone: 1B9H2 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66555-6-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 66555-6-Ig (same clone as 66555-6-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.2 μg Ki-67 Monoclonal antibody (66555-6-Ig, Clone:1B9H2,red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005), Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 66555-6-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50237-3

  Cytometric bead array standard curve of MP50237-3, KI67 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66555-5-PBS. Detection antibody: 66555-6-PBS. Standard:Ag26266. Range: 0.098-50 ng/mL.

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Informations sur le produit

66555-6-PBS cible Ki-67 dans les applications de IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Ki-67 Protéine recombinante Ag26266
• Nom complet: antigen identified by monoclonal antibody Ki-67
• Masse moléculaire calculée: 359 kDa
• Numéro d’acquisition GenBank: NM_002417
• Symbole du gène: KI67
• Identification du gène (NCBI): 4288
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

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