Anticorps Recombinant de lapin anti-Ki-67

Ki-67 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241499B1

N° de cat : 84192-3-PBS

Synonymes

KI67, MKI67, 241499B1, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67

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Galerie de données de validation

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  WB analysis of MCF-7 using 84192-3-RR (same clone as 84192-3-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 84192-3-RR (Ki-67 antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 84192-3-RR (same clone as 84192-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84192-3-RR (Ki-67 antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovarian cancer using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovarian cancer using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human malignant melanoma using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human malignant melanoma using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84192-3-RR (same clone as 84192-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IF Staining of human lung cancer using 84192-3-RR (same clone as 84192-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IF Staining of human lung cancer using 84192-3-RR (same clone as 84192-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IF Staining of human thyroid cancer using 84192-3-RR (same clone as 84192-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human thyroid cancer tissue using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IF Staining of human thyroid cancer using 84192-3-RR (same clone as 84192-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human thyroid cancer tissue using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 84192-3-RR (same clone as 84192-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 84192-3-RR (same clone as 84192-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 84192-3-RR (same clone as 84192-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 84192-3-RR (same clone as 84192-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 84192-3-RR (same clone as 84192-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IF Staining of hTERT-RPE1 using 84192-3-RR (same clone as 84192-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84192-3-RR (same clone as 84192-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

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Informations sur le produit

84192-3-PBS cible Ki-67 dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: antigen identified by monoclonal antibody Ki-67
• Masse moléculaire calculée: 359 kDa
• Numéro d’acquisition GenBank: NM_002417
• Symbole du gène: KI67
• Identification du gène (NCBI): 4288
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

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