Anticorps Polyclonal de lapin anti-Ki-67

• Phare
• Validé par KD/KO

Ki-67 Polyclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

Publications(1)

N° de cat : CL594-27309

Synonymes

KI67, MKI67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Ki 67

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Galerie de données de validation

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  IF Staining of HeLa using CL594-27309

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 Ki-67 antibody (CL594-27309) at dilution of 1:200, CL488-Phalloidin (green).

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  IF Staining of HeLa using CL594-27309

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 KI67 antibody (CL594-27309) at dilution of 1:200.

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  IF Staining of HeLa using CL594-27309

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 KI67 antibody (CL594-27309) at dilution of 1:200.

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  FC experiment of HeLa using CL594-27309

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human KI67 (CL594-27309) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of Ramos using CL594-27309

  1x10^6 Ramos cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human KI67 (CL594-27309)(red), or 0.8 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Applications testées

• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules Ramos, cellules HeLa

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL594-27309 cible Ki-67 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Ki-67 Protéine recombinante Ag26266
• Nom complet: antigen identified by monoclonal antibody Ki-67
• Masse moléculaire calculée: 359 kDa
• Numéro d’acquisition GenBank: NM_002417
• Symbole du gène: KI67
• Identification du gène (NCBI): 4288
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

Publications

Species	Application	Title
human	IF	Oncol Rep; Zinc finger protein 180 induces an apoptotic phenotype by activating METTL14 transcriptional activity in colorectal cancer; Authors - Liang Xu

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