Anticorps Polyclonal de lapin anti-MRPP3

MRPP3 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 20959-1-AP

Synonymes

KIAA0391, Protein only RNase P catalytic subunit, PRORP, Mitochondrial ribonuclease P catalytic subunit, EC:3.1.26.5

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Galerie de données de validation

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  WB analysis of mouse brain using 20959-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20959-1-AP (MRPP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20959-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20959-1-AP (MRPP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 20959-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20959-1-AP (MRPP3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20959-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20959-1-AP (MRPP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 20959-1-AP

  Immunofluorescent analysis of HepG2 cells, using KIAA0391 antibody 20959-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 20959-1-AP

  Immunofluorescent analysis of HepG2 cells, using KIAA0391 antibody 20959-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules L02, tissu hépatique de souris
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

20959-1-AP cible MRPP3 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MRPP3 Protéine recombinante Ag15196
• Nom complet: KIAA0391
• Masse moléculaire calculée: 583 aa, 67 kDa
• Poids moléculaire observé: 65-67 kDa
• Numéro d’acquisition GenBank: BC032221
• Symbole du gène: MRPP3
• Identification du gène (NCBI): 9692
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MRPP3 (Mitochondrial ribonuclease P protein 3), originally known as KIAA0391, has been identified as a member of human mitochondrial RNase P (mtRNase P), the endonuclease responsible for tRNA 5' end maturation by removing extra nucleotides from tRNA precursors at their 5' termini. Recently Daniel et al. reported that Rnase P and other mitochondrial RNA processing enzymes are localized near mt DNA nucleoids where they can initiate process of primary transcripts. (24703694)

Publications

Species	Application	Title
human	WB	Cell Metab; Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids.; Authors - Daniel F Bogenhagen

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