Anticorps Polyclonal de lapin anti-KIAA0528

KIAA0528 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21138-1-PBS

Synonymes

CDP138, C2CD5, C2 domain-containing protein 5, C2 domain-containing phosphoprotein of 138 kDa

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Galerie de données de validation

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  WB analysis of HeLa using 21138-1-AP (same clone as 21138-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21138-1-PBS in a different storage buffer formulation.

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  WB analysis using 21138-1-AP (same clone as 21138-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21138-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 21138-1-AP (same clone as 21138-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21138-1-AP (KIAA0528 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21138-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 21138-1-AP (same clone as 21138-1-PBS)

  IP result of anti-KIAA0528 (IP:21138-1-AP, 4ug; Detection:21138-1-AP 1:700) with HeLa cells lysate 520ug. This data was developed using the same antibody clone with 21138-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 21138-1-AP (same clone as 21138-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21138-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 21138-1-AP (same clone as 21138-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21138-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 21138-1-AP (same clone as 21138-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using KIAA0528 antibody 21138-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 21138-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21138-1-PBS cible KIAA0528 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KIAA0528 Protéine recombinante Ag15590
• Nom complet: KIAA0528
• Masse moléculaire calculée: 1000 aa, 110 kDa
• Poids moléculaire observé: 110-125 kDa
• Numéro d’acquisition GenBank: BC042498
• Symbole du gène: KIAA0528
• Identification du gène (NCBI): 9847
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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