Anticorps Recombinant de lapin anti-KIAA0776

KIAA0776 Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230363G7

N° de cat : 83047-5-PBS

Synonymes

230363G7, E3 UFM1 protein ligase 1, E3 UFM1-protein ligase 1, E3 UFM1-protein transferase 1, EC:2.3.2.-

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Galerie de données de validation

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  IF Staining of HepG2 using 83047-5-RR (same clone as 83047-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KIAA0776 antibody (83047-5-RR, Clone: 230363G7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83047-5-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83047-5-RR (same clone as 83047-5-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human KIAA0776 (83047-5-RR, Clone:230363G7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83047-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83047-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83047-5-RR against Human KIAA0776 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83047-5-PBS cible KIAA0776 dans les applications de IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: KIAA0776 Protéine recombinante Ag23362
• Nom complet: KIAA0776
• Numéro d’acquisition GenBank: BC036379
• Symbole du gène: KIAA0776
• Identification du gène (NCBI): 23376
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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