Anticorps Recombinant de lapin anti-KIAA1217

• Phare
• Validé par KD/KO

KIAA1217 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240276F3

N° de cat : 83361-2-RR

Synonymes

SKT, Sickle tail protein homolog, 240276F3

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Galerie de données de validation

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  WB analysis of HEK-293 using 83361-2-RR

  WB result of KIAA1217 antibody (83361-2-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIAA1217 transfected HEK-293 cells.

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  WB analysis using 83361-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83361-2-RR (KIAA1217 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of MCF-7 using 83361-2-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug KIAA1217 Recombinant antibody (83361-2-RR, Clone:240276F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83361-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83361-2-RR against Human KIAA1217 were performed. The affinity constant is 1.46 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83361-2-RR cible KIAA1217 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: KIAA1217 Protéine recombinante Ag19367
• Nom complet: KIAA1217
• Masse moléculaire calculée: 1943 aa, 214 kDa
• Poids moléculaire observé: 100-110, 140-160, 210
• Numéro d’acquisition GenBank: BC018764
• Symbole du gène: KIAA1217
• Identification du gène (NCBI): 56243
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KIAA1217, sickle tail protein homolog, is required for normal development of intervertebral disks. It promotes epithelial-mesenchymal transition and hepatocellular carcinoma metastasis by interacting with and activating STAT3, indicating its potential as a therapeutic target for curbing HCC metastasis (PMID: 35008530). It is also a novel candidate gene associated with isolated and syndromic vertebral malformations (PMID: 32369272). KIAA1217 has 9 alternatively spliced isoforms of 100-110 kDa, 140-160 kDa, 210 kDa.

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