Anticorps Monoclonal anti-KIAA1429

KIAA1429 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3F1A4

N° de cat : 68235-1-PBS

Synonymes

VIRMA, 3F1A4, MSTP054, virilizer

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Galerie de données de validation

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  WB analysis using 68235-1-Ig (same clone as 68235-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68235-1-Ig (KIAA1429 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68235-1-PBS in a different storage buffer formulation.

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  WB analysis using 68235-1-Ig (same clone as 68235-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68235-1-Ig (KIAA1429 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68235-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 68235-1-Ig (same clone as 68235-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68235-1-Ig (KIAA1429 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68235-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 68235-1-Ig (same clone as 68235-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human KIAA1429 (68235-1-Ig, Clone:3F1A4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68235-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68235-1-PBS cible KIAA1429 dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: KIAA1429 Protéine recombinante Ag22449
• Nom complet: KIAA1429
• Masse moléculaire calculée: 1812 aa, 202 kDa
• Poids moléculaire observé: 200-210 kDa
• Numéro d’acquisition GenBank: BC113380
• Symbole du gène: KIAA1429
• Identification du gène (NCBI): 25962
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

VIRMA (KIAA1429) is a key component of m6A methyltransferase (writer) complex that include METTL3, METTL14, WTAP, VIRMA, HAKAI, ZC3H13, and RBM15. VIRMA mediates methylation in the 3'UTR and around the stop codon, thus affecting alternative polyadenylation. VIRMA has been reported to act as an oncogenic factor in breast cancer and liver cancer. KIAA1429 exhibits differential nuclear and cytoplasmic distribution in colorectal cancer samples, and high cytoplasmic expression of KIAA1429 is associated with poor response to chemotherapy. BRAF-mediated phosphorylation of KIAA1429 facilitates its aggregation in the cytoplasm. Furthermore, cytoplasmic KIAA1429 promotes WNT pathway activation by binding and stabilizing FZD7, thereby further enhancing cancer stemness and oxaliplatin resistance (PMID: 40611274).

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