Anticorps Polyclonal de lapin anti-KIDINS220

• Phare
• Validé par KD/KO

KIDINS220 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(9)

Avis (1)

N° de cat : 21856-1-AP

Synonymes

Ankyrin repeat-rich membrane-spanning protein, ARMS, KIDINS 220, Kinase D-interacting substrate of 220 kDa

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Galerie de données de validation

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  WB analysis of HeLa using 21856-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21856-1-AP (KIDINS220 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21856-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21856-1-AP (KIDINS220 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21856-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21856-1-AP (KIDINS220 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 21856-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21856-1-AP (KIDINS220 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 21856-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21856-1-AP (KIDINS220 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21856-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21856-1-AP (KIDINS220 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 21856-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21856-1-AP (KIDINS220 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 21856-1-AP

  IP result of anti-KIDINS220 (IP:21856-1-AP, 4ug; Detection:21856-1-AP 1:3000) with HEK-293 cells lysate 1800 ug.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Raji, tissu cérébral humain
• Résultats positifs en IP: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

21856-1-AP cible KIDINS220 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KIDINS220 Protéine recombinante Ag16448
• Nom complet: kinase D-interacting substrate, 220kDa
• Masse moléculaire calculée: 1672 aa, 187 kDa
• Poids moléculaire observé: 186-197 kDa
• Numéro d’acquisition GenBank: BC130610
• Symbole du gène: KIDINS220
• Identification du gène (NCBI): 57498
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Kidins220 (Kinase D-interacting substrate 220 kD) is a crucial mediator of signal transduction in neural tissues. Also it named ARMS(Ankyrin Repeat-Rich Membrane Spanning) due to the presence of ankyrin repeats and transmembrane domains together with other binding motifs. It is a multidomain transmembrane protein that not only transduces signaling mediated by the receptor tyrosine kinase (RTK), but also regulates the stability of cytoskeletal network through a number of cytoskeleton-associated proteins, including Septin5, MAPs , and stathmin family members. KIDINS220 exists some isoforms with MV 194 kDa, 185 kDa, 115 kDa, and 60 kDa.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport.; Authors - Steinberg Florian F
human	WB	J Exp Med; Kidins220/ARMS binds to the B cell antigen receptor and regulates B cell development and activation.; Authors - Gina J Fiala ; KD Validated
mouse	WB	Sci Adv; Kidins220 and Aiolos promote thymic iNKT cell development by reducing TCR signals; Authors - Laurenz A Herr
human	WB	Nat Commun; Regulation of the endosomal SNX27-retromer by OTULIN.; Authors - Aurelia Stangl
human	WB,IP	Proc Natl Acad Sci U S A; A unique PDZ domain and arrestin-like fold interaction reveals mechanistic details of endocytic recycling by SNX27-retromer.; Authors - Matthew Gallon
human		Front Immunol; Patients' IgLON5 autoantibodies interfere with IgLON5-protein interactions; Authors - Jon Landa

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kincaid (Verified Customer) (06-02-2024)

H4 cell lysate run on SDS-PAGE gel. aKIDINS220 diluted in 3% BSA in TBS-tween (0.1%).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H4 cells

KIDINS220 Antibody Western Blot validation (1:1000 dilution) in H4 cells (Cat no:21856-1-AP)

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