Anticorps Polyclonal de lapin anti-KIF14

KIF14 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 26000-1-AP

Synonymes

KIAA0042, KIF14, kinesin family member 14, Kinesin like protein KIF14

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Galerie de données de validation

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  WB analysis of T-47D using 26000-1-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 26000-1-AP (KIF14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26000-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26000-1-AP (KIF14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 26000-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 26000-1-AP (KIF14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293T using 26000-1-AP

  IP result of anti-KIF14 (IP:26000-1-AP, 4ug; Detection:26000-1-AP 1:300) with HEK-293T cells lysate 4000 ug.

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  IHC staining of human prostate cancer using 26000-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26000-1-AP (KIF14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 26000-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26000-1-AP (KIF14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules T-47D, cellules HEK-293T, cellules HeLa
• Résultats positifs en IP: cellules HEK-293T,
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

26000-1-AP cible KIF14 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KIF14 Protéine recombinante Ag23219
• Nom complet: kinesin family member 14
• Numéro d’acquisition GenBank: BC113742
• Symbole du gène: KIF14
• Identification du gène (NCBI): 9928
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IHC	J Thorac Dis; Exploring diverse programmed cell-death patterns to develop a novel gene signature for predicting the prognosis of lung adenocarcinoma patients; Authors - Zhanming Ma
human	WB,IF	J Cell Mol Med; BUB1/KIF14 complex promotes anaplastic thyroid carcinoma progression by inducing chromosome instability; Authors - Tiefeng Jin
human	IHC	Pharmaceuticals (Basel); Identification of Key Immune and Cell Cycle Modules and Prognostic Genes for Glioma Patients through Transcriptome Analysis; Authors - Kaimin Guo
human	IHC	Cell Death Discov; Identification of tRF-29-79MP9P9NH525 as a biomarker and tumor suppressor of gastric cancer via regulating KIF14/AKT pathway; Authors - Jiaxin Ge
human	IHC	Carcinogenesis; NUF2 and NEK2 promote malignant progression of gallbladder cancer by remodeling the extracellular matrix; Authors - Ming Gao
human	WB	BMC Urol; KIF14 plays a role in the regulation of the cell cycle and has implications for prognosis in clear cell renal cell carcinoma; Authors - Jie Wang

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