Anticorps Polyclonal de lapin anti-KIF15

• Phare
• Validé par KD/KO

KIF15 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30774-1-PBS

Synonymes

hKLP2, Kinesin-like protein 2, Kinesin-like protein 7, Kinesin-like protein KIF15, KLP2

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Galerie de données de validation

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  WB analysis using 30774-1-AP (same clone as 30774-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30774-1-AP (KIF15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30774-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 30774-1-AP (same clone as 30774-1-PBS)

  WB result of KIF15 antibody (30774-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF15 transfected HeLa cells. This data was developed using the same antibody clone with 30774-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 30774-1-AP (same clone as 30774-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using KIF15 antibody (30774-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30774-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30774-1-PBS cible KIF15 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KIF15 Protéine recombinante Ag33897
• Nom complet: kinesin family member 15
• Masse moléculaire calculée: 160 kDa
• Poids moléculaire observé: 145-150 kDa
• Numéro d’acquisition GenBank: NM_020242
• Symbole du gène: KIF15
• Identification du gène (NCBI): 56992
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. It is located in the cytoplasm and biased expression in testis. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15 (14618103). It is known that KIF15 participates in important events during neuronal development and involves multiple cellular processes such as proliferation, apoptosis, and differentiation. There is emerging evidence indicating that KIF15 plays an important role in several malignancies including pancreatic cancer, hepatocellular carcinoma, and breast cancer (PMID: 33277366).

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