Anticorps Recombinant de lapin anti-KIF15

• Phare
• Validé par KD/KO

KIF15 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230049F1

N° de cat : 82903-1-PBS

Synonymes

230049F1, hKLP2, Kinesin-like protein 2, Kinesin-like protein 7, Kinesin-like protein KIF15

---

Galerie de données de validation

• 

  WB analysis using 82903-1-RR (same clone as 82903-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82903-1-RR (KIF15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82903-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 82903-1-RR (same clone as 82903-1-PBS)

  WB result of KIF15 antibody (82903-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF15 transfected HeLa cells. This data was developed using the same antibody clone with 82903-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 82903-1-RR (same clone as 82903-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82903-1-RR (KIF15 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82903-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82903-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82903-1-RR against Human KIF15 were performed. The affinity constant is 75.8 pM.

---

Informations sur le produit

82903-1-PBS cible KIF15 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: KIF15 Protéine recombinante Ag33897
• Nom complet: kinesin family member 15
• Masse moléculaire calculée: 160 kDa
• Poids moléculaire observé: 160 kDa
• Numéro d’acquisition GenBank: NM_020242
• Symbole du gène: KIF15
• Identification du gène (NCBI): 56992
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15. (14618103)

{{ptg:RelatedPrimaryAntibodies}}