Anticorps Monoclonal anti-KIF20A

• Phare
• Validé par KD/KO

KIF20A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2A8E3

N° de cat : 67190-1-PBS

Synonymes

2A8E3, FLJ21151, GG10_2, Kinesin-like protein KIF20A, Mitotic kinesin-like protein 2

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Galerie de données de validation

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  WB analysis using 67190-1-Ig (same clone as 67190-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67190-1-Ig (KIF20A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67190-1-Ig (same clone as 67190-1-PBS)

  WB result of KIF20A antibody (67190-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF20A transfected HeLa cells. This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67190-1-Ig (same clone as 67190-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67190-1-Ig (KIF20A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67190-1-Ig (same clone as 67190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67190-1-Ig (KIF20A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67190-1-Ig (same clone as 67190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67190-1-Ig (KIF20A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67190-1-Ig (same clone as 67190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67190-1-Ig (KIF20A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67190-1-Ig (same clone as 67190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67190-1-Ig (KIF20A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67190-1-Ig (same clone as 67190-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KIF20A antibody (67190-1-Ig, Clone: 2A8E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67190-1-PBS cible KIF20A dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: KIF20A Protéine recombinante Ag8916
• Nom complet: kinesin family member 20A
• Masse moléculaire calculée: 890 aa, 100 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC012999
• Symbole du gène: KIF20A
• Identification du gène (NCBI): 10112
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

KIF20A, also known as RAB6KIFL or MKlp2, is a kinesin originally identified as a binding partner for the rab6 GTPase involved in protein transport at the Golgi apparatus. KIF20A belongs to a large family of motor proteins that accumulate in mitotic cells and is required for normal cell division. KIF20A is thought to be involved in carcinogenesis. In normal tissues KIF20A is almost undetectable while it is overexpressed in various cancers. KIF20A has been identified as a novel promising candidate for anticancer immunotherapy.

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