Anticorps Polyclonal de lapin anti-KIF21A

KIF21A Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27276-1-PBS

Synonymes

KIF2, KIAA1708, FEOM3A, FEOM1, CFEOM1

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Galerie de données de validation

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  WB analysis using 27276-1-AP (same clone as 27276-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27276-1-AP (KIF21A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27276-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 27276-1-AP (same clone as 27276-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27276-1-AP (KIF21A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27276-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 27276-1-AP (same clone as 27276-1-PBS)

  IP result of anti-KIF21A (IP:27276-1-AP, 4ug; Detection:27276-1-AP 1:500) with mouse brain tissue lysate 5600ug. This data was developed using the same antibody clone with 27276-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 27276-1-AP (same clone as 27276-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27276-1-AP (KIF21A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27276-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 27276-1-AP (same clone as 27276-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27276-1-AP (KIF21A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27276-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27276-1-PBS cible KIF21A dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KIF21A Protéine recombinante Ag26192
• Nom complet: kinesin family member 21A
• Masse moléculaire calculée: 187 kDa
• Poids moléculaire observé: 190-200 kDa
• Numéro d’acquisition GenBank: NM_001173463
• Symbole du gène: KIF21A
• Identification du gène (NCBI): 55605
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

KIF21A is well known for its association with congenital fibrosis of the extraocular muscles type 1 (CFEOM1). CFEOM1-related mutations in KIF21A relieve autoinhibition of the motor and third coiled-coil stalk, which results in enhanced accumulation of KIF21A in axonal growth cones and aberrant axon morphology in the oculomotor nerve.(PMID: 38767486)

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