Anticorps Polyclonal de lapin anti-KIF23

• Phare
• Validé par KD/KO

KIF23 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(8)

Avis (1)

N° de cat : 28587-1-AP

Synonymes

MKLP1, KNSL5

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Galerie de données de validation

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  WB analysis using 28587-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28587-1-AP (KIF23 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 28587-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28587-1-AP (KIF23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28587-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28587-1-AP (KIF23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IF Staining of HepG2 using 28587-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KIF23 antibody (28587-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MG U-87
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

28587-1-AP cible KIF23 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KIF23 Protéine recombinante Ag29346
• Nom complet: kinesin family member 23
• Masse moléculaire calculée: 110 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: NM_138555
• Symbole du gène: KIF23
• Identification du gène (NCBI): 9493
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KIF23, also known as KNSL5 and MKLP1, is a member of the kinesin-like protein family. It plays a crucial role in cytokinesis, the final stage of cell division. It is involved in the formation of the central spindle, which is essential for the proper separation of the cytoplasm and the formation of the cleavage furrow. KIF23 is also involved in the organization and transport of intracellular vesicles and organelles. Mutations in KIF23 have been associated with congenital dyserythropoietic anemia type III(PMID: 23570799).

Publications

Species	Application	Title
		Cell Biosci; TPM2 attenuates progression of prostate cancer by blocking PDLIM7-mediated nuclear translocation of YAP1; Authors - Zonglong Wu
human	IF	PLoS Biol; The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.; Authors - Umut Batman
human	WB,IHC	Front Cell Dev Biol; Identification of KIF23 as a Prognostic Biomarker Associated With Progression of Clear Cell Renal Cell Carcinoma.; Authors - Zonglong Wu ; KD Validated
human	WB	Front Immunol; HBx Mediated Increase of DDX17 Contributes to HBV-Related Hepatocellular Carcinoma Tumorigenesis.; Authors - Mei-Ling Dong
human	WB	Toxicol Appl Pharmacol; Kinesin family member 23 exerts a protumor function in breast cancer via stimulation of the Wnt/β-catenin pathway.; Authors - Xin He ; KD Validated
	WB	Cell Rep; Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.; Authors - Ana C Almeida ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wissem (Verified Customer) (07-30-2024)

antibody works well to detect KIF23 in U2OS cell extracts.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10,000

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