Anticorps Recombinant de lapin anti-KIF5B

• Phare
• Validé par KD/KO

KIF5B Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B18

N° de cat : 82485-1-PBS

Synonymes

KNS1, KNS, KINH, Kinesin-1 heavy chain, KIF 5B

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Galerie de données de validation

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  WB analysis using 82485-1-RR (same clone as 82485-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82485-1-RR (KIF5B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82485-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82485-1-RR (same clone as 82485-1-PBS)

  WB result of KIF5B antibody (82485-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF5B transfected HeLa cells. This data was developed using the same antibody clone with 82485-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 82485-1-RR (same clone as 82485-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82485-1-RR (KIF5B antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82485-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82485-1-RR (same clone as 82485-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KIF5B antibody (82485-1-RR, Clone: 2B18 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82485-1-PBS in a different storage buffer formulation.

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Informations sur le produit

82485-1-PBS cible KIF5B dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: KIF5B Protéine recombinante Ag16250
• Nom complet: kinesin family member 5B
• Masse moléculaire calculée: 963 aa, 110 kDa
• Poids moléculaire observé: 110-120 kDa
• Numéro d’acquisition GenBank: BC126279
• Symbole du gène: KIF5B
• Identification du gène (NCBI): 3799
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

KIF5B, also known as KINH, KNS1, belongs to the Kinesin-1 family. KIF5B contains three structural domains: a globular N-terminal motor domain, a central alpha-helical rod domain, and a globular C-terminal domain. During the G2 phase of the cell cycle in a BICD2-dependent manner, KIF5B antagonizes the dynein function and drives the separation of nuclei and centrosomes (PMID: 20386726). KIF5B drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells (KIF5B:24088571).

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