Anticorps Polyclonal de lapin anti-KIF7

• Phare
• Validé par KD/KO

KIF7 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 24693-1-AP

Synonymes

KIF7, kinesin family member 7, Kinesin like protein KIF7, UNQ340

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Galerie de données de validation

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  WB analysis of HEK-293 using 24693-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24693-1-AP (KIF7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24693-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24693-1-AP (KIF7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 24693-1-AP

  IP result of anti-KIF7 (IP:24693-1-AP, 5ug; Detection:24693-1-AP 1:500) with HEK-293 cells lysate 1760ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293,
• Résultats positifs en IP: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

24693-1-AP cible KIF7 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KIF7 Protéine recombinante Ag19119
• Nom complet: kinesin family member 7
• Masse moléculaire calculée: 1343 aa, 151 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC112271
• Symbole du gène: KIF7
• Identification du gène (NCBI): 374654
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KIF7(Kinesin family member 7) is a microtubule interacting protein that functions to regulate Hh signaling by maintaining the architecture of the primary cilium, a complex microtubule-based organelle with many signal transduction functions (PMID: 26439735). KIF7 has been shown to have both negative and positive roles in Shh signal transduction. Without a ligand, KIF7 localizes to the cilium base where it forms a complex with Gli proteins and other pathway components and promotes the processing of GliRs (PMID: 21552264).

Publications

Species	Application	Title
human	IF	Sci Adv; Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.; Authors - Chuan Chen
human	WB	Cells; Determination of WWOX Function in Modulating Cellular Pathways Activated by AP-2α and AP-2γ Transcription Factors in Bladder Cancer.; Authors - Damian Kołat
human	WB	Thorac Cancer; Human papillomavirus 16 (HPV 16) E6 but not E7 inhibits the antitumor activity of LKB1 in lung cancer cells by downregulating the expression of KIF7.; Authors - Yue Hu ; KD Validated
human	WB,IHC	Cell Death Dis; Circular RNA circUBE2J2 acts as the sponge of microRNA-370-5P to suppress hepatocellular carcinoma progression.; Authors - Lu Zhang
human	WB	Proteome Sci; Proteomics identifies differentially expressed proteins in glioblastoma U87 cells treated with hederagenin; Authors - Yesen Zhang
mouse,human	WB,IF	Leukemia; P4HA2 hydroxylates SUFU to regulate the paracrine Hedgehog signaling and promote B-cell lymphoma progression; Authors - Quanfu Li

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