Anticorps Recombinant de lapin anti-KIR2DL3/CD158b2

KIR2DL3/CD158b2 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

242282E11

N° de cat : 98373-3-RR

Synonymes

KIR2DL3, Killer cell immunoglobulin-like receptor 2DL3, GL183, CD158B2, CD158b

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Galerie de données de validation

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  FC experiment of human PBMCs using 98373-3-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human KIR2DL3/CD158b2 Rabbit RecAb (98373-3-RR, Clone: 242282E11) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human NCAM1/CD56. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98373-3-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human KIR2DL3/CD158b2 Rabbit RecAb (98373-3-RR, Clone: 242282E11) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98373-3-RR cible KIR2DL3/CD158b2 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 3
• Masse moléculaire calculée: 38 kDa
• Numéro d’acquisition GenBank: NM_015868.2
• Symbole du gène: KIR2DL3
• Identification du gène (NCBI): 3804
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

Killer cell immunoglobulin-like receptors (KIRs) are a diverse family of inhibitory and activating receptors expressed on NK cells and a subset of T cells (PMID: 11861603). These polymorphic receptors interact with specific motifs on HLA class I molecules, modulate NK cytolytic activity and are encoded by genes located on chromosome 19q13.4 (PMID: 17069649). KIR2DL3, also known as CD158B2 or NKAT2, is an inhibitory receptor that is specific for HLA-C alleles (HLA-Cw1, HLA-Cw3 and HLA-Cw7) (PMID: 10196125). It is a 341-amino acid transmembrane glycoprotein consisting of an extracellular region containing two C2-type Ig-like domains, a 19-amino acid hydrophobic transmembrane region, and a long cytoplasmic tail with two immunoreceptor tyrosine-based inhibitory motifs (ITIMs). KIR2DL3 inhibits the cytolytic activity of NK cells thus preventing cell lysis (PMID: 7724594).

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