Anticorps Polyclonal de lapin anti-KISS1

• Phare
• Validé par KD/KO

KISS1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 18375-1-PBS

Synonymes

KiSS 1, KiSS 1 metastasis suppressor, Kisspeptin 1, Kisspeptin-1, Metastasis suppressor KiSS 1

---

Galerie de données de validation

• 

  WB analysis using 18375-1-AP (same clone as 18375-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18375-1-AP (KISS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 18375-1-AP (same clone as 18375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18375-1-AP (KISS1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 18375-1-AP (same clone as 18375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18375-1-AP (KISS1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 18375-1-AP (same clone as 18375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18375-1-AP (KISS1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 18375-1-AP (same clone as 18375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18375-1-AP (KISS1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 18375-1-AP (same clone as 18375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18375-1-AP (KISS1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 18375-1-PBS

  Immunofluorescent analysis of NIH/3T3 cells using 18375-1-AP (KISS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Informations sur le produit

18375-1-PBS cible KISS1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KISS1 Protéine recombinante Ag13240
• Nom complet: KiSS-1 metastasis-suppressor
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC022819
• Symbole du gène: KISS1
• Identification du gène (NCBI): 3814
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

KISS1, also known as kisspeptin, is a metastasis suppressor protein that suppresses metastases of human melanomas and breast carcinomas without affecting tumorigenicity. It may regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A C-terminally amidated peptide of KISS1, metastin (kisspeptin-54 or kp-54), functions as the endogenous ligand of the G-protein coupled receptor GPR54 (PMID: 11457843; 11385580). KISS1-GPR54 signaling is involved in the regulation of reproduction, playing an important role in initiating secretion of gonadotropin-releasing hormone (GnRH) at puberty (PMID: 18753370).

{{ptg:RelatedPrimaryAntibodies}}