Anticorps Polyclonal de lapin anti-KISS1R-Specific

KISS1R-Specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15505-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse cerebellum using 15505-1-AP (same clone as 15505-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 15505-1-AP (same clone as 15505-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 15505-1-AP (same clone as 15505-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 15505-1-AP (same clone as 15505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 15505-1-AP (same clone as 15505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 15505-1-AP (same clone as 15505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 15505-1-AP (same clone as 15505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 15505-1-AP (same clone as 15505-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using KISS1R antibody 15505-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15505-1-AP (same clone as 15505-1-PBS)

  Immunofluorescent analysis of Hela cells, using KISS1R antibody 15505-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 15505-1-AP (same clone as 15505-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using KISS1R antibody 15505-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 15505-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15505-1-PBS cible KISS1R-Specific dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: KISS1 receptor
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 75 kDa, 43-45 kDa
• Numéro d’acquisition GenBank: NM_032551
• Symbole du gène: KISS1R
• Identification du gène (NCBI): 84634
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

KISS1R is the receptor for metastin (kisspeptin-54, 13, 14, 10), C- terminally amidated peptides of KISS-1. KISS-1 is a metastasis suppressor protein that suppresses metastases in malignant melanomas and in some other carcinomas without affecting tumorigenicity. The metastasis suppressor properties may be mediated in part by cell cycle arrest and induction of apoptosis in malignant cells. The receptor is also probably involved in the regulation and fine-tuning of trophoblast invasion generated by the trophoblast itself. Analysis of the transduction pathways activated by the receptor identifies coupling to phospholipase C and intracellular calcium release through pertussis toxin-insensitive G(q) proteins.

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