Anticorps Polyclonal de lapin anti-c-Kit/CD117

c-Kit/CD117 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

22

N° de cat : 18696-1-AP

Synonymes

C Kit, CD117, c-Kit, c-Kit/CD117, KIT, PBT, Proto oncogene c Kit, SCFR, Tyrosine protein kinase Kit

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Galerie de données de validation

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  WB analysis of Mo7e using 18696-1-AP

  Mo7e cells were subjected to SDS PAGE followed by western blot with 18696-1-AP (c-Kit/CD117 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of stromal tumor using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 18696-1-AP (c-Kit/CD117 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of stromal tumor using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 18696-1-AP (c-Kit/CD117 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of Insulinoma using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 18696-1-AP (c-Kit/CD117 antibody) at dilution of 1:2000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of Insulinoma using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 18696-1-AP (c-Kit/CD117 antibody) at dilution of 1:2000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human placenta using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18696-1-AP (KIT antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human testis using 18696-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18696-1-AP (KIT antibody) at dilution of 1:400 (under 40x lens).

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  IF Staining of MCF-7 using 18696-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 18696-1-AP (c-Kit/CD117 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 18696-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug c-Kit/CD117 antibody (18696-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: Mo7e cells,
• Résultats positifs en IHC: tissu stroma tumoral, tissu d'insulinome, tissu placentaire humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 8 publications below
• IF: See 7 publications below

Informations sur le produit

18696-1-AP cible c-Kit/CD117 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• Masse moléculaire calculée: 110 kDa
• Poids moléculaire observé: 140-160 kDa
• Numéro d’acquisition GenBank: BC071593
• Symbole du gène: KIT
• Identification du gène (NCBI): 3815
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KIT, also named as SCFR, c-Kit and CD117, is a transmembrane tyrosine kinase encoded by the c-Kit proto oncogene. It is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand(SCF) binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Phosphorylation at tyrosine 721 of KIT allows binding and activation of PI3 kinase. Loss of expression of KIT appears to be associated with progression of some tumors (melanoma) and autocrine/paracrine stimulation of the kit/SCF system may participate in human solid tumors such as lung, breast, testicular and gynecological malignancies. Mutations in Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer.

Publications

Species	Application	Title
human	WB	Mech Ageing Dev; Acceleration of melanocyte senescence by the proinflammatory cytokines IFNγ and TNFα impairs the repigmentation response of vitiligo patients to narrowband ultraviolet B (NBUVB) phototherapy; Authors - Bing-Qi Dong
human	IHC	Front Cell Dev Biol; Single-Cell Sequencing Unveils the Heterogeneity of Nonimmune Cells in Chronic Apical Periodontitis.; Authors - Xinwei Lin
human	IHC	Front Immunol; Immune Infiltrating Cells-Derived Risk Signature Based on Large-scale Analysis Defines Immune Landscape and Predicts Immunotherapy Responses in Glioma Tumor Microenvironment.; Authors - Nan Zhang
human	IF	J Exp Clin Cancer Res; Growth differentiation factor 15 contributes to marrow adipocyte remodeling in response to the growth of leukemic cells.; Authors - Wei Lu
mouse	IF	Andrology; Exposure to elevated temperature affects the expression of piwi-interacting RNAs and associated transcripts in mouse testes; Authors - Wenbin Chen
human	WB	Tumour Biol; Orai1 mediates tumor-promoting store-operated Ca(2+) entry in human gastrointestinal stromal tumors via c-KIT and the extracellular signal-regulated kinase pathway.; Authors - Lei Wang

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