Anticorps Polyclonal de lapin anti-KLF15

• Phare
• Validé par KD/KO

KLF15 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 13749-1-AP

Synonymes

KKLF, KLF15, Krueppel like factor 15, Kruppel like factor 15

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Galerie de données de validation

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  WB analysis using 13749-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13749-1-AP (KLF15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13749-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13749-1-AP (KLF15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules 3T3-L1, cellules C2C12, cellules MDA-MB-231, tissu cardiaque de souris, tissu hépatique de souris, tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

13749-1-AP cible KLF15 dans les applications de WB, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KLF15 Protéine recombinante Ag4690
• Nom complet: Kruppel-like factor 15
• Masse moléculaire calculée: 416 aa, 44 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC036733
• Symbole du gène: KLF15
• Identification du gène (NCBI): 28999
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KLF15, a kidney-enriched Krüppel-like zinc finger-containing transcription factors, is a transcriptonal activator that binds the CLCNKA promoter. The nuclear protein KLF15, is expressed primarily in liver, heart, skeletal muscle and kidney tissues but is not detected in lymphoid tissues or bone marrow. It is an important regulator of GLUT4 in both adipose and muscle tissues.Although the calculated molecular mass of KLF15 is 44 kDa, the apparent molecular mass on SDS-PAGE is 65 kDa due to the post-translational modifications (PMID:10982849,PMID:16615998).

Publications

Species	Application	Title
rat	WB,ChIP	Cell Metab; Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle.; Authors - Noriaki Shimizu
human	WB	Mol Cell Biol; A remodeled Hsp90 molecular chaperone ensemble with the novel co-chaperone Aarsd1 is required for muscle differentiation.; Authors - Pablo C Echeverría
mouse	WB,IF	Epigenomics; Multi-omics insights into the pathogenesis of diabetic cardiomyopathy: epigenetic and metabolic profiles; Authors - Li Zhou
mouse	WB	PLoS One; PMID: 24167585; Authors - Jung Dae Young DY ; KO Validated

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