Anticorps Monoclonal anti-KLF5

• Phare
• Validé par KD/KO

KLF5 Monoclonal Antibody for Indirect ELISA, WB

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

2C11C5

N° de cat : 66850-1-PBS

Synonymes

Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2, Basic transcription element-binding protein 2

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Galerie de données de validation

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  WB analysis using 66850-1-Ig (same clone as 66850-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66850-1-Ig (KLF5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66850-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66850-1-Ig (same clone as 66850-1-PBS)

  WB result of KLF5 antibody (66850-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KLF5 transfected HeLa cells. This data was developed using the same antibody clone with 66850-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66850-1-Ig (same clone as 66850-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human KLF5 (66850-1-Ig, Clone:2C11C5) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66850-1-PBS in a different storage buffer formulation.

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Applications publiées

• IHC: See 1 publications below

Informations sur le produit

66850-1-PBS cible KLF5 dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: KLF5 Protéine recombinante Ag15403
• Nom complet: Kruppel-like factor 5 (intestinal)
• Masse moléculaire calculée: 457 aa, 51 kDa
• Poids moléculaire observé: 51-57 kDa
• Numéro d’acquisition GenBank: BC042131
• Symbole du gène: KLF5
• Identification du gène (NCBI): 688
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling.

Publications

Species	Application	Title
human	IHC	Adv Sci (Weinh); Positive Feedback Regulation between KLF5 and XPO1 Promotes Cell Cycle Progression of Basal like Breast Cancer; Authors - Yu Tang

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