Anticorps Recombinant de lapin anti-KLF9

KLF9 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230436H2

N° de cat : 83183-4-RR

Synonymes

GC-box-binding protein 1, BTE-binding protein 1, BTEB1, BTEB, Basic transcription element-binding protein 1

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Galerie de données de validation

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  WB analysis using 83183-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83183-4-RR (KLF9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of A549 using 83183-4-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug KLF9 Recombinant antibody (83183-4-RR, Clone:230436H2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83183-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83183-4-RR against Human KLF9 were performed. The affinity constant is 0.767 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HepG2, cellules K-562, cellules U2OS
• Résultats positifs en FC (Intra): cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83183-4-RR cible KLF9 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: KLF9 Protéine recombinante Ag34411
• Nom complet: Kruppel-like factor 9
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 27-35 kDa
• Numéro d’acquisition GenBank: BC074880
• Symbole du gène: KLF9
• Identification du gène (NCBI): 687
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Krüppel-like factor 9 (KLF9) was a member of SP/KLF family of DNA-binding transcriptional regulators featured by a highly homologous C-terminal three C2H2-type zinc finger DNA-binding domains. KLF9 has been previously reported to be downregulated in various types of human malignancy and serves as a tumour suppressor at tumour onset and development, affecting cell proliferation, apoptosis, metastasis and tumorigenicity (PMID: 32855660).

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