Anticorps Polyclonal de lapin anti-KLHL14-Specific

• Phare
• Validé par KD/KO

KLHL14-Specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14849-1-PBS

Synonymes

Protein interactor of Torsin-1A, Printor, KLHL14, KIAA1384, Kelch-like protein 14

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Galerie de données de validation

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  WB analysis of HeLa using 14849-1-AP (same clone as 14849-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 14849-1-AP (same clone as 14849-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 14849-1-AP (same clone as 14849-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 14849-1-AP (same clone as 14849-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 14849-1-AP (same clone as 14849-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 14849-1-AP (same clone as 14849-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse ovary using 14849-1-AP (same clone as 14849-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 14849-1-AP (same clone as 14849-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 14849-1-AP (same clone as 14849-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 14849-1-AP (same clone as 14849-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 14849-1-AP (same clone as 14849-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 14849-1-AP (same clone as 14849-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 14849-1-AP (same clone as 14849-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 14849-1-AP (same clone as 14849-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 14849-1-AP (same clone as 14849-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 14849-1-AP (same clone as 14849-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14849-1-AP (same clone as 14849-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KLHL14-Specific antibody (14849-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14849-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14849-1-PBS cible KLHL14-Specific dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: kelch-like 14 (Drosophila)
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 39-45 kDa
• Numéro d’acquisition GenBank: NM_020805
• Symbole du gène: KLHL14
• Identification du gène (NCBI): 57565
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

KLHL14, a member of the Kelch-like gene family, has six Kelch domains and is localized to the endoplasmic reticulum. KLHL14, a novel tumor suppressor, is a subunit of a ubiquitin ligase that associates with the endoplasmic reticulum-associated protein degradation (ERAD) machinery, regulating of B cell receptor-dependent NF-κB signaling (PMID: 32127472). KLHL14 has two isoforms of 70kDa and 43kDa.

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