Anticorps Polyclonal de lapin anti-KLHL2

KLHL2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14449-1-PBS

Synonymes

Kelch-like protein 2, Actin-binding protein Mayven, ABP KELCH

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Galerie de données de validation

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  WB analysis of MCF-7 using 14449-1-AP (same clone as 14449-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14449-1-AP (KLHL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14449-1-PBS in a different storage buffer formulation.

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  WB analysis using 14449-1-AP (same clone as 14449-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14449-1-AP (KLHL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14449-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 14449-1-AP (same clone as 14449-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 14449-1-AP (KLHL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14449-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 14449-1-AP (same clone as 14449-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using KLHL2 antibody (14449-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14449-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14449-1-PBS cible KLHL2 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KLHL2 Protéine recombinante Ag5950
• Nom complet: kelch-like 2, Mayven (Drosophila)
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC022503
• Symbole du gène: KLHL2
• Identification du gène (NCBI): 11275
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

KLHL2 contains an SH3-binding domain in the N terminus and a POZ domain and 6 tandem repeats of approximately 50 amino acids each in the C terminus. In astrocytoma/glioblastoma cells, KLHL2 colocalized with actin filaments in the cytoskeleton remodeling regions in stress fibers and in patchy cortical actin-rich regions of the cell margins. In primary rat hippocampal neurons, it was highly expressed in the cell body and in neurite processes(PMID: 10397770).

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