Anticorps Polyclonal de lapin anti-KLRG1

KLRG1 Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10974-1-PBS

Synonymes

MAFA 2F1, MAFA, Killer cell lectin-like receptor subfamily G member 1, ITIM-containing receptor MAFA-L, C-type lectin domain family 15 member A

---

Galerie de données de validation

• 

  WB analysis using 10974-1-AP (same clone as 10974-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10974-1-AP (KLRG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10974-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human peripheral blood leukocyte using 10974-1-AP (same clone as 10974-1-PBS)

  Human peripheral blood leukocytes were subjected to SDS PAGE followed by western blot with 10974-1-AP (KLRG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10974-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 10974-1-AP (same clone as 10974-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 10974-1-AP (KLRG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10974-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 10974-1-AP (same clone as 10974-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10974-1-AP (KLRG1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10974-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 10974-1-AP (same clone as 10974-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10974-1-AP (KLRG1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10974-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

10974-1-PBS cible KLRG1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KLRG1 Protéine recombinante Ag1407
• Nom complet: killer cell lectin-like receptor subfamily G, member 1
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC012621
• Symbole du gène: KLRG1
• Identification du gène (NCBI): 10219
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

KLRG1 (killer cell lectin-like receptor subfamily G, member 1) is a C-type lectin inhibitory receptor that contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain. KLRG1 is expressed by subsets of NK and T cells, existing both as a monomer and as a disulfide-linked homodimer (PMID: 10741410; 19604491). It is considered to be a cell differentiation marker for NK and T cells and is strongly induced by viral and other infections (PMID: 20373518). Through interactions with members of the cadherin family, KLRG1 plays an inhibitory role in NK- and T-cell function (PMID: 19009530; 20373518). Because of the glycosylation and disulfide bonds of the KLRG1 proteins, the observed molecular weights are typically 30-38 kDa (PMID: 17404256).

{{ptg:RelatedPrimaryAntibodies}}