Anticorps Monoclonal anti-KMO

KMO Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

2D2F2

Avis (1)

N° de cat : 60029-1-Ig

Synonymes

2D2F2, EC:1.14.13.9, Kynurenine 3 hydroxylase, Kynurenine 3 monooxygenase, Kynurenine 3-hydroxylase

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Galerie de données de validation

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  WB analysis using 60029-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60029-1-Ig (KMO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules SKOV-3

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

60029-1-Ig cible KMO dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: KMO Protéine recombinante Ag1109
• Nom complet: kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)
• Masse moléculaire calculée: 486 aa, 56 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC005297
• Symbole du gène: KMO
• Identification du gène (NCBI): 8564
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KMO(Kynurenine 3-monooxygenase) is a membrane protein located on the outer membrane of mitochondria. Tissue distribution studies have revealed that, in rats, highest enzyme activity is found in kidney and liver, with brain having the least activity in comparison to peripheral organs(PMID: 9237672).

Publications

Species	Application	Title
mouse,human	IF	J Am Soc Nephrol; Loss of Kynurenine 3-Mono-oxygenase Causes Proteinuria.; Authors - Ron Korstanje
human	IHC	Neuro Oncol; Molecular imaging correlates of tryptophan metabolism via the kynurenine pathway in human meningiomas.; Authors - Edit Bosnyák
human	IHC	Oncol Lett; Viability of diffuse large B-cell lymphoma cells is regulated by kynurenine 3-monooxygenase activity; Authors - NANAKA MORITA
mouse	WB	Hum Mol Genet; Retention of stress susceptibility in the mdx mouse model of Duchenne muscular dystrophy after PGC-1α overexpression or ablation of IDO1 or CD38; Authors - Erynn E Johnson
mouse	IHC	Br J Pharmacol; Chronic stress induces behavioural changes through increased kynurenic acid by downregulation of kynurenine-3-monooxygenase with microglial decline; Authors - Masaya Hasegawa
rat	WB	J Pharmacol Sci; Thrombin-induced kynurenine 3-monooxygenase causes variations in the kynurenine pathway, leading to neurological deficits in a murine intracerebral hemorrhage model; Authors - Masatoshi Ohnishi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hala (Verified Customer) (06-07-2022)

Works very good

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: Huh6 and Hepg2

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