Anticorps Recombinant de lapin anti-KMO

KMO Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240586D3

N° de cat : 83812-1-RR

Synonymes

Kynurenine 3-monooxygenase, Kynurenine 3-hydroxylase, Kynurenine 3 monooxygenase, Kynurenine 3 hydroxylase, EC:1.14.13.9

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Galerie de données de validation

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  WB analysis of MCF-7 using 83812-1-RR

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 83812-1-RR (KMO antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 83812-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83812-1-RR (KMO antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 83812-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83812-1-RR (KMO antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 83812-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83812-1-RR (KMO antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 83812-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83812-1-RR (KMO antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of MCF-7 using 83812-1-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug KMO Recombinant antibody (83812-1-RR, Clone:240586D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83812-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83812-1-RR against Human KMO were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules MCF-7,
• Résultats positifs en IHC: tissu rénal de souris, tissu cardiaque de souris, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83812-1-RR cible KMO dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: KMO Protéine recombinante Ag35029
• Nom complet: kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)
• Masse moléculaire calculée: 486 aa, 56 kDa
• Poids moléculaire observé: 52-56 kDa
• Numéro d’acquisition GenBank: BC005297
• Symbole du gène: KMO
• Identification du gène (NCBI): 8564
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KMO(Kynurenine 3-monooxygenase) is an NADPH-dependent flavin monooxygenase, catalysing the hydroxylation of the L-kynurenine to form L-3-hydroxykynurenine. KMO is a membrane protein located on the outer membrane of mitochondria. Tissue distribution studies have revealed that, in rats, highest enzyme activity is found in kidney and liver, with brain having the least activity in comparison to peripheral organs(PMID: 9237672).

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