Anticorps Recombinant de lapin anti-KMT2C

KMT2C Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240542A2

N° de cat : 83595-4-RR

Synonymes

MLL3, 240542A2, HALR, Homologous to ALR protein, KIAA1506

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Galerie de données de validation

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  IF Staining of MCF-7 using 83595-4-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KMT2C antibody (83595-4-RR, Clone: 240542A2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (green).

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  FC experiment of U2OS using 83595-4-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug KMT2C Recombinant antibody (83595-4-RR, Clone:240542A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83595-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83595-4-RR against Human KMT2C were performed. The affinity constant is 2.67 nM.

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Applications testées

• Résultats positifs en IF/ICC: cellules MCF-7,
• Résultats positifs en FC (Intra): cellules U2OS,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83595-4-RR cible KMT2C dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: KMT2C Protéine recombinante Ag28875
• Nom complet: myeloid/lymphoid or mixed-lineage leukemia 3
• Masse moléculaire calculée: 541 kDa
• Numéro d’acquisition GenBank: NM_170606
• Symbole du gène: KMT2C
• Identification du gène (NCBI): 58508
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Lysine (K)-specific methyltransferase 2C (KMT2C, also known as MLL3) belongs to the mixed-lineage leukemia (MLL) family of histone methyltransferases which methylate the histone 3 tail at lysine 4 (H3K4) as part of the complex proteins associated with Set 1 (COMPASS) complex. Although originally identified as oncogenic fusions in leukemia, genome-wide mutation studies have revealed frequent, presumably loss-of-function, mutations in various members of the MLL family, including MLL2/KMT2B, MLL3/KMT2C, and MLL4/KMT2D in a variety of malignancies, particularly solid tumors. Mechanistic studies of KMT2C in normal cells have focused primarily on its role in enhancer regulation by deposition of H3K4me1 marks.

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