Anticorps Monoclonal anti-KPNA3

• Phare
• Validé par KD/KO

KPNA3 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1D3D2

Avis (1)

N° de cat : 67892-1-Ig

Synonymes

hSRP1, Importin alpha Q2, Importin subunit alpha 3, IPOA4, Karyopherin subunit alpha 3, KPNA3, Qip2, SRP1, SRP1 gamma, SRP1gamma, SRP4

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Galerie de données de validation

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  WB analysis using 67892-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67892-1-Ig (KPNA3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HepG2 using 67892-1-Ig

  WB result of KPNA3 antibody (67892-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KPNA3 transfected HepG2 cells.

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  WB analysis using 67892-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67892-1-Ig (KPNA3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IHC staining of mouse testis using 67892-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67892-1-Ig (KPNA3 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 67892-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67892-1-Ig (KPNA3 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules PC-12
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1500-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67892-1-Ig cible KPNA3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: KPNA3 Protéine recombinante Ag27767
• Nom complet: karyopherin alpha 3 (importin alpha 4)
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC017355
• Symbole du gène: KPNA3
• Identification du gène (NCBI): 3839
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

karyopherin subunit alpha 3 (KPNA3) is a member of the importin alpha family which can transport molecules between the mucleus and cytoplasm. It is ubiquitous expressed in brain,testis and esophagus. KPNA3 is associated with many severe diseases such as hepatocellular Carcinoma (PMID: 31417635) , schizophrenia, major depression and opiate dependence (PMID: 22960338) . The molecular weight of KPNA3 is about 58 kDa.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Daulet (Verified Customer) (02-09-2022)

It worked on the lysates of SH-SY5Y cells. So, if you are going to use it on this sample, then I can recommend it. However, bear in mind, that whether it will work or not depends on how you treat your samples and other protocol niceties.

• Applications: Western Blot
• Cell Tissue Type: SH-SY5Y