Anticorps Recombinant de lapin anti-KPNA3

KPNA3 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

251681E1

N° de cat : 86722-1-RR

Synonymes

Importin alpha Q2, Importin subunit alpha-4, Karyopherin subunit alpha 3, Karyopherin subunit alpha-3, Qip2

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Galerie de données de validation

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  WB analysis using 86722-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86722-1-RR (KPNA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86722-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86722-1-RR (KPNA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Caco-2, cellules HeLa, cellules HSC-T6, cellules LNCaP, cellules NIH/3T3, cellules PC-12

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86722-1-RR cible KPNA3 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: KPNA3 Protéine recombinante Ag27755
• Nom complet: karyopherin alpha 3 (importin alpha 4)
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 57-60 kDa
• Numéro d’acquisition GenBank: BC017355
• Symbole du gène: KPNA3
• Identification du gène (NCBI): 3839
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KPNA3, karyopherin subunit alpha 3, also known as SRP1 or IPOA4. It is expected to be located in cytoplasm and nucleus, the protein is ubiquitous and highly expressed in heart, skeletal muscle. The calculated molecular weight of KPNA3 is 57 kDa. Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin.

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