Anticorps Polyclonal de lapin anti-KPNA4

• Phare
• Validé par KD/KO

KPNA4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 12463-1-AP

Synonymes

Importin subunit alpha-3, IPOA3, Karyopherin subunit alpha-4, KPNA 4, QIP1

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Galerie de données de validation

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  WB analysis of A549 using 12463-1-AP

  WB result of KPNA4 antibody (12463-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KPNA4 transfected A549 cells.

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  WB analysis using 12463-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12463-1-AP (KPNA4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12463-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12463-1-AP (KPNA4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 12463-1-AP

  IP result of anti-KPNA4 (IP:12463-1-AP, 4ug; Detection:12463-1-AP 1:600) with A549 cells lysate 2000ug.

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  IHC staining of mouse brain using 12463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12463-1-AP (KPNA4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human bowen disease using 12463-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12463-1-AP (KPNA4 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12463-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12463-1-AP (KPNA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 12463-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using KPNA4 antibody (12463-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules Jurkat, cellules NIH/3T3, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: cellules A549
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

12463-1-AP cible KPNA4 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KPNA4 Protéine recombinante Ag3133
• Nom complet: karyopherin alpha 4 (importin alpha 3)
• Masse moléculaire calculée: 521 aa, 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC034493
• Symbole du gène: KPNA4
• Identification du gène (NCBI): 3840
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KPNA4, also named importin subunit alpha-3, karyopherin-alpha4, is a cytoplasmic protein that recognizes nuclear localization signals (NLSs) and dock NLS-containing proteins to the nuclear pore complex. Nuclear import, mediated in part by karyopherin-α (KPNA)/importin-α subtypes, regulates transcription factor access to the genome and determines cell fate. KPNA4-mediated nuclear transport of Ras-responsive element-binding protein (RREB1), which sustains Ras/ERK pathway signaling through repressing miR-143/145 expression (PMID: 31822798). KPNA4 is one of the main isoforms that is activated in many human cancers. KPNA4 expression was elevated in head and neck of squamous cell carcinoma (PMID: 33188837).

Publications

Species	Application	Title
human	WB,CoIP	Virol Sin; African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway.; Authors - Yisha Zhuo
human	WB	Oncogene; The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer.; Authors - Kai Li ; KD Validated
mouse	WB	J Neurosci; The subcellular distribution of HDAC1 in neurotoxic conditions is dependent on serine phosphorylation.; Authors - Yunjiao Zhu
mouse	WB	Exp Gerontol; Age-dependent changes in nuclear-cytoplasmic signaling in skeletal muscle.; Authors - Shama R Iyer
human	WB	Tumour Biol; Reanalysis of microRNA expression profiles identifies novel biomarkers for hepatocellular carcinoma prognosis.; Authors - Zhengqiang Wang
human	WB	Viruses; Ivermectin Inhibits HBV Entry into the Nucleus by Suppressing KPNA2; Authors - Anna Nakanishi

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