Anticorps Recombinant de lapin anti-KPNA4

• Phare
• Validé par KD/KO

KPNA4 Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230098H3

N° de cat : 82898-1-PBS

Synonymes

230098H3, Importin subunit alpha-3, IPOA3, Karyopherin subunit alpha-4, KPNA 4

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Galerie de données de validation

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  WB analysis using 82898-1-RR (same clone as 82898-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82898-1-RR (KPNA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82898-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 82898-1-RR (same clone as 82898-1-PBS)

  WB result of KPNA4 antibody (82898-1-RR; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KPNA4 transfected A549 cells. This data was developed using the same antibody clone with 82898-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 82898-1-RR (same clone as 82898-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KPNA4 antibody (82898-1-RR, Clone: 230098H3 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82898-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82898-1-RR (same clone as 82898-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.13 ug Anti-Human KPNA4 (82898-1-RR, Clone:230098H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.13 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82898-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82898-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82898-1-RR against Human KPNA4 were performed. The affinity constant is 0.417 nM.

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Informations sur le produit

82898-1-PBS cible KPNA4 dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: KPNA4 Protéine recombinante Ag3133
• Nom complet: karyopherin alpha 4 (importin alpha 3)
• Masse moléculaire calculée: 521 aa, 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC034493
• Symbole du gène: KPNA4
• Identification du gène (NCBI): 3840
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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