Anticorps Recombinant de lapin anti-KPNA4

• Phare
• Validé par KD/KO

KPNA4 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230098H1

N° de cat : 82898-2-PBS

Synonymes

QIP1, KPNA 4, Karyopherin subunit alpha-4, IPOA3, Importin subunit alpha-3

---

Galerie de données de validation

• 

  WB analysis using 82898-2-RR (same clone as 82898-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82898-2-RR (KPNA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82898-2-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 82898-2-RR (same clone as 82898-2-PBS)

  WB result of KPNA4 antibody (82898-2-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KPNA4 transfected A549 cells. This data was developed using the same antibody clone with 82898-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 82898-2-RR (same clone as 82898-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82898-2-RR (KPNA4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82898-2-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 82898-2-RR (same clone as 82898-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KPNA4 antibody (82898-2-RR, Clone: 230098H1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82898-2-PBS in a different storage buffer formulation.

• 

  FC experiment of A549 using 82898-2-RR (same clone as 82898-2-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug KPNA4 Recombinant antibody (82898-2-RR, Clone:230098H1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82898-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82898-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82898-2-RR against Human KPNA4 were performed. The affinity constant is 0.678 nM.

---

Informations sur le produit

82898-2-PBS cible KPNA4 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: KPNA4 Protéine recombinante Ag3133
• Nom complet: karyopherin alpha 4 (importin alpha 3)
• Masse moléculaire calculée: 521 aa, 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC034493
• Symbole du gène: KPNA4
• Identification du gène (NCBI): 3840
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

KPNA4, also named importin subunit alpha-3, karyopherin-alpha4, is a cytoplasmic protein that recognizes nuclear localization signals (NLSs) and dock NLS-containing proteins to the nuclear pore complex. Nuclear import, mediated in part by karyopherin-α (KPNA)/importin-α subtypes, regulates transcription factor access to the genome and determines cell fate. KPNA4-mediated nuclear transport of Ras-responsive element-binding protein (RREB1), which sustains Ras/ERK pathway signaling through repressing miR-143/145 expression (PMID: 31822798). KPNA4 is one of the main isoforms that is activated in many human cancers. KPNA4 expression was elevated in head and neck of squamous cell carcinoma (PMID: 33188837).

{{ptg:RelatedPrimaryAntibodies}}