Anticorps Polyclonal de lapin anti-KPTN

• Phare
• Validé par KD/KO

KPTN Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 16094-1-AP

Synonymes

2E4, Actin associated protein 2E4, Kaptin, kaptin (actin binding protein), KPTN

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Galerie de données de validation

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  WB analysis using 16094-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16094-1-AP (KPTN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16094-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16094-1-AP (KPTN antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16094-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16094-1-AP (KPTN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 16094-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16094-1-AP (KPTN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human cerebellum using 16094-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 16094-1-AP (KPTN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 16094-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16094-1-AP (KPTN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 16094-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16094-1-AP (KPTN antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 16094-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16094-1-AP (KPTN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules NIH/3T3, tissu de cervelet humain, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque humain, tissu cutané humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 9 publications below

Informations sur le produit

16094-1-AP cible KPTN dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KPTN Protéine recombinante Ag8785
• Nom complet: kaptin (actin binding protein)
• Masse moléculaire calculée: 436 aa, 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC009249
• Symbole du gène: KPTN
• Identification du gène (NCBI): 11133
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Kaptin, also named as KPTN and Actin-associated protein 2E4, originally isolated from human blood platelets and likely to be involved in the actin rearrangements occurring during activation. It plays a unique role in the actin rearrangements that accompany platelet activation and stereocilia formation. Kaptin is necessary for normal neuromorphogenesis. It may play a role in producing the sensory apparatus in hair cells.

Publications

Species	Application	Title
human	WB	Nature; Structure of the nutrient-sensing hub GATOR2.; Authors - Max L Valenstein
human	WB	Nature; Architecture of the human GATOR1 and GATOR1-Rag GTPases complexes.; Authors - Kuang Shen
human	WB	Nature; KICSTOR recruits GATOR1 to the lysosome and is necessary for nutrients to regulate mTORC1.; Authors - Rachel L Wolfson ; KD Validated
human	WB	Cell Rep; CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth; Authors - Shasha Yin ; KO Validated
human	WB	Cells; The SZT2 Interactome Unravels New Functions of the KICSTOR Complex.; Authors - Cecilia Cattelani
human	WB	Nat Metab; Dihydroxyacetone phosphate signals glucose availability to mTORC1.; Authors - Jose M Orozco