Anticorps Polyclonal de lapin anti-KRI1

KRI1 Polyclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF,ELISA

Conjugaison

Non conjugué

N° de cat : 16243-1-AP

Synonymes

KRI1, KRI1 homolog (S. cerevisiae), Protein KRI1 homolog

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Galerie de données de validation

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  WB analysis of HEK-293 using 16243-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16243-1-AP (KRI1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16243-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16243-1-AP (KRI1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16243-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16243-1-AP (KRI1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 16243-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16243-1-AP (KRI1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16243-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16243-1-AP (KRI1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16243-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16243-1-AP (KRI1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 16243-1-AP

  Immunofluorescent analysis of HepG2 cells using 16243-1-AP (KRI1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

16243-1-AP cible KRI1 dans les applications de WB, IF,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KRI1 Protéine recombinante Ag9171
• Nom complet: KRI1 homolog (S. cerevisiae)
• Masse moléculaire calculée: 709 aa, 80 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC009876
• Symbole du gène: KRI1
• Identification du gène (NCBI): 65095
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.