Anticorps Recombinant de lapin anti-Cytokeratin 13

• Phare
• Validé par KD/KO

Cytokeratin 13 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

230366B10

N° de cat : 83058-1-PBS

Synonymes

KRT13, 230366B10, CK 13, CK13, CK-13

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Galerie de données de validation

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  WB analysis using 83058-1-RR (same clone as 83058-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83058-1-RR (KRT13 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 83058-1-RR (same clone as 83058-1-PBS)

  WB result of Cytokeratin 13 antibody (83058-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytokeratin 13 transfected A431 cells. This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00074-2

  Sandwich ELISA standard curve of MP00074-2, Human Cytokeratin 13 Recombinant Matched Antibody Pair - PBS only. 83058-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0217. 83058-5-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  Sample test of MP00074-2

  The mean Cytokeratin 13 concentration was determined to be 28.00 ng/mL in A431 cell extract based on a 1.50 mg/mL extract load and 12.84 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load.

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  IHC staining of human urothelial carcinoma using 83058-1-RR (same clone as 83058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 83058-1-RR (same clone as 83058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 83058-1-RR (same clone as 83058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 83058-1-RR (same clone as 83058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical squamous cancer using 83058-1-RR (same clone as 83058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical squamous cancer using 83058-1-RR (same clone as 83058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

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  IHC staining of rat skin using 83058-1-RR (same clone as 83058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skin using 83058-1-RR (same clone as 83058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 83058-1-RR (same clone as 83058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 83058-1-RR (same clone as 83058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse skin using 83058-1-RR (same clone as 83058-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 13 antibody (83058-1-RR, Clone: 230366B10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse skin using 83058-1-RR (same clone as 83058-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 13 antibody (83058-1-RR, Clone: 230366B10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

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  IF Staining of HaCaT using 83058-1-RR (same clone as 83058-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Cytokeratin 13 antibody (83058-1-RR, Clone: 230366B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83058-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83058-1-RR against Human Cytokeratin 13 were performed. The affinity constant is 1.99 nM.

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Informations sur le produit

83058-1-PBS cible Cytokeratin 13 dans les applications de WB, IHC, IF/ICC, IF-P, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cytokeratin 13 Protéine recombinante Ag0217
• Nom complet: keratin 13
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC002661
• Symbole du gène: Cytokeratin 13
• Identification du gène (NCBI): 3860
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Keratin 13 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in keratin 13 gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2.

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