Anticorps Polyclonal de lapin anti-Cytokeratin 17

Cytokeratin 17 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

N° de cat : 18502-1-AP

Synonymes

39.1, Cell and organelle markers, CK 17, Cytokeratin 17, Cytoskeleton Marker, K17, keratin 17, KRT17, PCHC1

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Galerie de données de validation

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  WB analysis of HeLa using 18502-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 18502-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18502-1-AP

  IP Result of anti-Cytokeratin 17 (IP:18502-1-AP, 4ug; Detection:18502-1-AP 1:600) with HeLa cells lysate 1600ug.

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  IHC staining of human cervical cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human cervical cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18502-1-AP (Cytokeratin 17 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 18502-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18502-1-AP (Cytokeratin 17 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 18502-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 17 antibody (18502-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 18502-1-AP

  Immunofluorescent analysis of HeLa cells using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 18502-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Cytokeratin 17 antibody (18502-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu cutané humain, tissu de cancer du côlon humain, tissu de cancer du pancréas humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

18502-1-AP cible Cytokeratin 17 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytokeratin 17 Protéine recombinante Ag13351
• Nom complet: keratin 17
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC011901
• Symbole du gène: KRT17
• Identification du gène (NCBI): 3872
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filaments that make up the cytoskeleton. Type I keratins are a group of acidic intermediate filament proteins and type II keratins are the basic or neutral counterparts. Keratin 17 is encoded by the KRT17 gene and is a type I cytokeratin found in nail beds, hair follicles, sebaceous glands, and epidermal appendages.

 What is the molecular weight of cytokeratin 17?

The molecular weight of cytokeratin 17 is approximately 49 kDa.

 What is the function of cytokeratin 17?

Cytokeratin 17 plays a role in the formation and maintenance of epidermal appendages, especially in determining the shape and orientation of hair. It is required for the maintenance of the anagen (growth) state of hair follicles by modulating the function of TNF-alpha for hair cycling and is also involved in tissue repair (PMID: 16702408).

 What is the cellular localization of cytokeratin 17?

Cytokeratin 17 expression is located in the cytoplasm.

 What is the tissue specificity of cytokeratin 17?

Cytokeratin 17 expression is found in the outer root sheath and medulla regions of the hair follicle, particularly in the eyebrow, beard, nail bed epithelium, basal cells of the oral epithelium, and sweat and mammary glands (PMID: 2451629). Expression can also be found in epithelial layers of the prostate and urinary bladder and cambial cells of the sebaceous gland at the protein level.

 What is the role of cytokeratin 17 in disease?

Mutations in the KRT17 gene are a cause of Jackson-Lawler type pachyonychia congenita type 2 (PC2); an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy (PMID: 7539673). Defects in KRT17 also lead to steatocystoma multiplex (SM) (PMID: 9767294). Cytokeratin 17 acts as an autoantigen in the immunopathogenesis of psoriasis (PMID: 23722817).