Anticorps Polyclonal de lapin anti-Cytokeratin 18

Cytokeratin 18 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 18708-1-AP

Synonymes

CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18

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Galerie de données de validation

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  WB analysis of A431 using 18708-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 18708-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18708-1-AP (Cytokeratin 18 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 18708-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 18708-1-AP

  IP Result of anti-Cytokeratin 18 (IP:18708-1-AP, 4ug; Detection:18708-1-AP 1:500) with A431 cells lysate 2000ug.

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  IHC staining of human renal cell carcinoma using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human kidney using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human endometrial cancer using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human endometrial cancer using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human nephroblastoma using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human nephroblastoma using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human nephroblastoma using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human nephroblastoma using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin cancer using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 18708-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 18708-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 18708-1-AP (Cytokeratin 18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules COLO 320, cellules HepG2
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: tissu de carcinome à cellules rénales humain, tissu de cancer de la peau humain, tissu de cancer de l'endomètre humain, tissu de cancer du côlon humain, tissu de cancer du sein humain, tissu de néphroblastome humain, tissu de tumeur ovarienne humain, tissu hépatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

18708-1-AP cible Cytokeratin 18 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: bovin, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: keratin 18
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC000180
• Symbole du gène: KRT18
• Identification du gène (NCBI): 3875
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. This antibody is specifically agianst KRT18.

Publications

Species	Application	Title
mouse	IHC	Oncoscience; The helix-loop-helix transcriptional regulator Id4 is required for terminal differentiation of luminal epithelial cells in the prostate.; Authors - Dhanushka Hewa Bostanthirige
bovine	IF	J Agric Food Chem; CIDEA Regulates De Novo Fatty Acid Synthesis in Bovine Mammary Epithelial Cells by Targeting the AMPK/PPARγ Axis and Regulating SREBP1; Authors - Ji Cheng
pig	IHC	Cells; Hypothalamic-Pituitary-Adrenal Hormones Impair Pig Fertilization and Preimplantation Embryo Development via Inducing Oviductal Epithelial Apoptosis: An In Vitro Study; Authors - Jin-Song An
bovine	IF	J Agric Food Chem; Mitochondrial Calcium Uniporter Regulator 1 (MCUR1) Relieves Mitochondrial Damage Induced by Lipopolysaccharide by Mediating Mitochondrial Ca2+ Homeostasis in Bovine Mammary Epithelial Cells; Authors - Xudong Sun