Anticorps Polyclonal de lapin anti-Cytokeratin 34

Cytokeratin 34 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 20449-1-AP

Synonymes

keratin 34, KRT34, keratin34, Keratin-34, KRT 34

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Galerie de données de validation

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  WB analysis of HeLa using 20449-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20449-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 20449-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20449-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 20449-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 20449-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 20449-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin cancer using 20449-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung squamous cell carcinoma using 20449-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung squamous cell carcinoma using 20449-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 20449-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin cancer using 20449-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 20449-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin cancer using 20449-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 20449-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 20449-1-AP (KRT34 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A375, cellules A431, cellules HepG2, cellules L02, tissu cutané de souris
• Résultats positifs en IHC: tissu de cancer de la peau humain, human lung squamous cell carcinoma tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

20449-1-AP cible Cytokeratin 34 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytokeratin 34 Protéine recombinante Ag14295
• Nom complet: keratin 34
• Masse moléculaire calculée: 436 aa, 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC041070
• Symbole du gène: KRT34
• Identification du gène (NCBI): 3885
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). KRT 34 is a type I keratin.

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