Anticorps Polyclonal de lapin anti-Cytokeratin 4

Cytokeratin 4 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 16572-1-AP

Synonymes

CK 4, CK4, CYK4, Cytokeratin 4, K4, keratin 4, KRT4, Type II keratin Kb4

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Galerie de données de validation

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  WB analysis of A431 using 16572-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16572-1-AP (Cytokeratin 4 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human bladder using 16572-1-AP

  human bladder tissue were subjected to SDS PAGE followed by western blot with 16572-1-AP (Cytokeratin 4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 16572-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16572-1-AP (Cytokeratin 4 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 16572-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16572-1-AP (Cytokeratin 4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 16572-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 4 antibody (16572-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

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  IF Staining of mouse skin using 16572-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using Cytokeratin 4 antibody (16572-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 16572-1-AP

  1X10^6 HeLa cells were stained with 0.2 ug Anti-Human Cytokeratin 4 (16572-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules A431, tissu vésical humain
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431, tissu cutané de souris
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Informations sur le produit

16572-1-AP cible Cytokeratin 4 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytokeratin 4 Protéine recombinante Ag9854
• Nom complet: keratin 4
• Masse moléculaire calculée: 534 aa, 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC042174
• Symbole du gène: KRT4
• Identification du gène (NCBI): 3851
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 4 is a type II cytokeratins. It is specifically found in differentiated layers of the mucosal and esophageal epithelia together with keratin 13.

Publications

Species	Application	Title
mouse	IF	Nature; A single-cell atlas of the airway epithelium reveals the CFTR-rich pulmonary ionocyte.; Authors - Lindsey W Plasschaert
	IF	Development; Novel dynamics of human mucociliary differentiation revealed by single-cell RNA sequencing of nasal epithelial cultures.; Authors - Sandra Ruiz García
human	IHC	Int J Mol Sci; Early Clinical Outcomes of the First Commercialized Human Autologous Ex Vivo Cultivated Oral Mucosal Epithelial Cell Transplantation for Limbal Stem Cell Deficiency: Two Case Reports and Literature Review; Authors - Hiroshi Toshida
human	IHC	Ann Diagn Pathol; The molecular-based differentiation of Heck's disease from its mimics including oral condyloma and white sponge nevus.; Authors - Ziv Schwartz
mouse	IF	Cell Rep; An Immunocompetent Mouse Model of HPV16(+) Head and Neck Squamous Cell Carcinoma.; Authors - Miranda B Carper
human	WB	Oncotarget; Significance of the NOR1-FOXA1/HDAC2-Slug regulatory network in epithelial-mesenchymal transition of tumor cells.; Authors - Wei Wang