Anticorps Polyclonal de lapin anti-Cytokeratin 8

• Phare
• Validé par KD/KO

Cytokeratin 8 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(11)

N° de cat : 10384-1-AP

Synonymes

KRT8, CARD2, CK 8, CK8, CK-8

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Galerie de données de validation

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  WB analysis using 10384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10384-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10384-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10384-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10384-1-AP

  IP result of anti-Cytokeratin 8 (IP:10384-1-AP, 4ug; Detection:10384-1-AP 1:1000) with HeLa cells lysate 2000ug.

• 

  IHC staining of human tonsillitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded normal colon slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10384-1-AP (Cytokeratin 8 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10384-1-AP (Cytokeratin 8 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 10384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10384-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 8 antibody (10384-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 10384-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Cytokeratin 8 antibody (10384-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of MCF-7 using 10384-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug Cytokeratin 8 Polyclonal antibody (10384-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules A549, cellules COLO 320, cellules HaCaT, cellules MCF-7, tissu cutané de rat, tissu cutané de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu d'appendicite humain, tissu de cancer du sein humain, tissu de carcinome à cellules rénales humain, tissu de côlon humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du sein humain,
• Résultats positifs en IF/ICC: cellules MCF-7, cellules HeLa
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:40000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Informations sur le produit

10384-1-AP cible Cytokeratin 8 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, chien, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytokeratin 8 Protéine recombinante Ag0488
• Nom complet: keratin 8
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC000654
• Symbole du gène: Cytokeratin 8
• Identification du gène (NCBI): 3856
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cytokeratin 8 (CK8) is a protein that belongs to the cytokeratin family, which is a group of structural proteins found in the intracytoplasmic cytoskeleton of epithelial cells. CK8 forms intermediate filaments within the cytoplasm of simple epithelial cells, typically as a dimer with cytokeratin 18 (CK18). These filaments provide a stabilizing framework that helps determine cell shape and enables cells to cope with mechanical stress. CK8/CK18 filaments also serve as a mesh of "paths" for signaling molecules, metabolites, and pathogens to travel within the cell in an organized manner. CK8 is expressed in various epithelial tissues and is particularly associated with glandular and simple epithelia. Its expression is also a significant marker in several types of cancer, including lung and esophageal cancer, where it can indicate tumor progression and poor prognosis.

Publications

Species	Application	Title
mouse	IF	Dev Cell; An integrated approach identifies the molecular underpinnings of murine anterior visceral endoderm migration; Authors - Shifaan Thowfeequ
human	WB,IF	Cell Mol Life Sci; Crip2 affects vascular development by fine-tuning endothelial cell aggregation and proliferation; Authors - Shuaiqi Yang
human	WB,IP,IF	J Cell Sci; SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network; Authors - Archan Chakraborty ; KD Validated
mouse	WB,IF	Front Cell Dev Biol; ESRRB Facilitates the Conversion of Trophoblast-Like Stem Cells From Induced Pluripotent Stem Cells by Directly Regulating CDX2.; Authors - Shuai Yu
human	WB,IF	J Cell Sci; SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network.; Authors - Archan Chakraborty ; KD Validated
pig,human,goat,dog	IHC	Anat Rec (Hoboken); Comparative study of the external auditory canal in humans and large mammals.; Authors - Jun Wang

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