Anticorps Polyclonal de lapin anti-Cytokeratin 8

Cytokeratin 8 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(26)

Avis (2)

N° de cat : 17514-1-AP

Synonymes

KRT8, CARD2, CK 8, CK8, CK-8

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Galerie de données de validation

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  WB analysis using 17514-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 17514-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 17514-1-AP (Cytokeratin 8 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 17514-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 17514-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 17514-1-AP (Cytokeratin 8 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 17514-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 17514-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 17514-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 17514-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human bowen disease using 17514-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 17514-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 17514-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 17514-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17514-1-AP (Cytokeratin 8 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 17514-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17514-1-AP (Cytokeratin 8 antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 17514-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17514-1-AP (Cytokeratin 8 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using 17514-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using Cytokeratin 8 antibody (17514-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 17514-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 8 (17514-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules C6, cellules HeLa, cellules MCF-7
• Résultats positifs en IHC: tissu d'appendicite humain, tissu de cancer de la prostate humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu testiculaire de souris,
• Résultats positifs en FC (Intra): cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 12 publications below
• IF: See 10 publications below

Informations sur le produit

17514-1-AP cible Cytokeratin 8 dans les applications de WB, IHC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: keratin 8
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: NM_002273
• Symbole du gène: Cytokeratin 8
• Identification du gène (NCBI): 3856
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo. This antibody is specifically against KRT8.

Publications

Species	Application	Title
mouse,human	IF	Nat Aging; Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer; Authors - Yifei Cheng
human	WB	Oncogene; The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer; Authors - Mengjie Li
human	WB	Clin Transl Med; Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.; Authors - An-Dong Liu
mouse	IHC	Sci Rep; Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators.; Authors - Shu Wei
human	IHC	Sci Rep; A method for the identification of lactate metabolism-related prognostic biomarkers and its validations in non-small cell lung cancer; Authors - Weiyang Yang
mouse	WB,IF	iScience; Cysteine Dioxygenase Regulates the Epithelial Morphogenesis of Mammary Gland via Cysteine Sulfinic Acid.; Authors - Jianjun Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Emma (Verified Customer) (11-29-2021)

Works very well by IF on FFPE tissue. Used at 1:50 with a Tris-EDTA antigen retrieval.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Prostate

Cytokeratin 8 Antibody Immunofluorescence validation (1:50 dilution) in Prostate (Cat no:17514-1-AP)

FH

Joleen (Verified Customer) (06-12-2019)

This antibody recognizes a band that matched with the expected molecular weight of KRT8.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

Cytokeratin 8 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:17514-1-AP)

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