Anticorps Monoclonal anti-KU70,XRCC6

KU70,XRCC6 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3C12D9

N° de cat : 66607-1-PBS

Synonymes

KU70, XRCC6, 3C12D9, 5'-deoxyribose-5-phosphate lyase Ku70, 5'-dRP lyase Ku70

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Galerie de données de validation

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  WB analysis using 66607-1-Ig (same clone as 66607-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

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  WB analysis using 66607-1-Ig (same clone as 66607-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66607-1-Ig (same clone as 66607-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66607-1-Ig (same clone as 66607-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66607-1-Ig (same clone as 66607-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66607-1-Ig (same clone as 66607-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66607-1-Ig (same clone as 66607-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66607-1-Ig (same clone as 66607-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66607-1-Ig (same clone as 66607-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66607-1-Ig (same clone as 66607-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66607-1-Ig (same clone as 66607-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66607-1-Ig (same clone as 66607-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66607-1-Ig (same clone as 66607-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 66607-1-Ig (same clone as 66607-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 66607-1-Ig (same clone as 66607-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse breast cancer using 66607-1-Ig (same clone as 66607-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using KU70,XRCC6 antibody (66607-1-Ig, Clone: 3C12D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse breast cancer using 66607-1-Ig (same clone as 66607-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using KU70,XRCC6 antibody (66607-1-Ig, Clone: 3C12D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66607-1-Ig (same clone as 66607-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KU70,XRCC6 antibody (66607-1-Ig, Clone: 3C12D9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66607-1-Ig (same clone as 66607-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KU70,XRCC6 antibody (66607-1-Ig, Clone: 3C12D9 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66607-1-Ig (same clone as 66607-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human KU70,XRCC6 (66607-1-Ig, Clone:3C12D9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66607-1-PBS cible KU70,XRCC6 dans les applications de WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: KU70,XRCC6 Protéine recombinante Ag21301
• Nom complet: X-ray repair complementing defective repair in Chinese hamster cells 6
• Masse moléculaire calculée: 75 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC008343
• Symbole du gène: KU70
• Identification du gène (NCBI): 2547
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.

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