Anticorps Monoclonal anti-KU70,XRCC6

KU70,XRCC6 Monoclonal Antibody for WB, IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

3C12D9

N° de cat : CL594-66607

Synonymes

KU70, XRCC6, CTC box-binding factor 75 kDa subunit, ATP-dependent DNA helicase II 70 kDa subunit, ATP-dependent DNA helicase 2 subunit 1

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Galerie de données de validation

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  WB analysis using CL594-66607

  Various lysates were subjected to SDS PAGE followed by western blot with CL594-66607 (KU70,XRCC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using CL594-66607

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 KU70,XRCC6 antibody (CL594-66607, Clone: 3C12D9 ) at dilution of 1:200.

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  IF Staining of HeLa using CL594-66607

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 KU70,XRCC6 antibody (CL594-66607, Clone: 3C12D9 ) at dilution of 1:600.

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  FC experiment of HepG2 using CL594-66607

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human KU70,XRCC6 (CL594-66607, Clone:3C12D9) (red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, cellules MCF-7
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66607 cible KU70,XRCC6 dans les applications de WB, IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: KU70,XRCC6 Protéine recombinante Ag21301
• Nom complet: X-ray repair complementing defective repair in Chinese hamster cells 6
• Masse moléculaire calculée: 75 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC008343
• Symbole du gène: KU70
• Identification du gène (NCBI): 2547
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.

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