Anticorps Polyclonal de lapin anti-KYNU

• Phare
• Validé par KD/KO

KYNU Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (1)

N° de cat : 11796-1-AP

Synonymes

KYNU, Kynureninase, L kynurenine hydrolase

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Galerie de données de validation

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  WB analysis of HeLa using 11796-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11796-1-AP (KYNU antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11796-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11796-1-AP (KYNU antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 11796-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11796-1-AP (KYNU antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11796-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11796-1-AP (KYNU antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11796-1-AP

  Immunofluorescent analysis of HepG2 cells, using KYNU antibody 11796-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 11796-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KYNU antibody (11796-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HepG2, tissu hépatique de rat
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

11796-1-AP cible KYNU dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KYNU Protéine recombinante Ag2365
• Nom complet: kynureninase (L-kynurenine hydrolase)
• Masse moléculaire calculée: 465aa,52 kDa; 307aa,35 kDa
• Poids moléculaire observé: 45-52 kDa
• Numéro d’acquisition GenBank: BC000879
• Symbole du gène: KYNU
• Identification du gène (NCBI): 8942
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KYNU(kynureninase) is also named as L-kynurenine hydrolase and belongs to the kynureninase family. It is a pyridoxal-5'-phosphate-(pyridoxal-P)-depe11dent enzyme that catalyses the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively(PMID:8706755). In SDS-PAGE, it can detect a 48 kDa band lower than the preidicted molecular weight of 52 kDa due to limited proteolysis of the enzyme which might occur during handling of the protein(PMID:8706755). This protein is a 95-kD kD homodimer predominantly located in the cytoplasm(PMID:9180257). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	IF	J Hazard Mater; Particulate matter-induced metabolic recoding of epigenetics in macrophages drives pathogenesis of chronic obstructive pulmonary disease; Authors - Myungkyung Noh
human	WB	Virol Sin; Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection.; Authors - Yingying Song
rat	WB	Chem Res Toxicol; Tryptophan Pathway-Targeted Metabolomics Study on the Mechanism and Intervention of Cisplatin-Induced Acute Kidney Injury in Rats.; Authors - Bei Tan
human	WB	Clin Exp Dermatol; Downregulation of kynureninase restrains cutaneous squamous cell carcinoma proliferation and represses the PI3K/AKT pathway.; Authors - C Ci ; KD Validated
human	WB	Adv Sci (Weinh); Tryptophan Metabolism Acts as a New Anti-Ferroptotic Pathway to Mediate Tumor Growth; Authors - Dong Liu ; KO Validated
human	WB,IHC	Oncogene; Glutathione peroxidase 2 knockdown suppresses gastric cancer progression and metastasis via regulation of kynurenine metabolism; Authors - Handong Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hala (Verified Customer) (03-29-2022)

it works good in BSA 5% dissolved in TBST

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: hela cells

KYNU Antibody Western Blot validation (1/1000 dilution) in hela cells (Cat no:11796-1-AP)