Anticorps Recombinant de lapin anti-KYNU

KYNU Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251437C8

N° de cat : 86555-4-PBS

Synonymes

EC:3.7.1.3, Kynureninase, L kynurenine hydrolase, L-kynurenine hydrolase

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Galerie de données de validation

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  WB analysis using 86555-4-RR (same clone as 86555-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86555-4-RR (KYNU antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86555-4-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 86555-4-RR (same clone as 86555-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using KYNU antibody (86555-4-RR, Clone: 251437C8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86555-4-PBS in a different storage buffer formulation.

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Informations sur le produit

86555-4-PBS cible KYNU dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: KYNU Protéine recombinante Ag2365
• Nom complet: kynureninase (L-kynurenine hydrolase)
• Masse moléculaire calculée: 465aa,52 kDa; 307aa,35 kDa
• Poids moléculaire observé: 45-52 kDa
• Numéro d’acquisition GenBank: BC000879
• Symbole du gène: KYNU
• Identification du gène (NCBI): 8942
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

KYNU(kynureninase) is also named as L-kynurenine hydrolase and belongs to the kynureninase family. It is a pyridoxal-5'-phosphate-(pyridoxal-P)-depe11dent enzyme that catalyses the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively(PMID:8706755). In SDS-PAGE, it can detect a 48 kDa band lower than the preidicted molecular weight of 52 kDa due to limited proteolysis of the enzyme which might occur during handling of the protein(PMID:8706755).

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