Anticorps Polyclonal de lapin anti-Cytokeratin 81

• Phare
• Validé par KD/KO

Cytokeratin 81 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11342-1-PBS

Synonymes

KRT81, Cytokeratin,Keratin, ghHb1, keratin 81, Keratin-81

---

Galerie de données de validation

• 

  WB analysis using 11342-1-AP (same clone as 11342-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11342-1-AP (same clone as 11342-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 11342-1-AP (same clone as 11342-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human spleen using 11342-1-AP (same clone as 11342-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 11342-1-AP (same clone as 11342-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 11342-1-AP (same clone as 11342-1-PBS)

  IP result of anti-KRT81 (IP:11342-1-AP, 4ug; Detection:11342-1-AP 1:300) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11342-1-AP (same clone as 11342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11342-1-AP (KRT81 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11342-1-AP (same clone as 11342-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KRT81 antibody (11342-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

11342-1-PBS cible Cytokeratin 81 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytokeratin 81 Protéine recombinante Ag1890
• Nom complet: keratin 81
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC021241
• Symbole du gène: KRT81
• Identification du gène (NCBI): 3887
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT81 is a type II keratin.

{{ptg:RelatedPrimaryAntibodies}}