Anticorps Monoclonal anti-Ki-67

Ki-67 Monoclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

1B9H2

N° de cat : 66555-6-Ig

Synonymes

KI67, MKI67, 1B9H2, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67

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Galerie de données de validation

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  IHC staining of human tonsillitis using 66555-6-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66555-6-Ig (Ki-67 antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66555-6-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66555-6-Ig (Ki-67 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 66555-6-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66555-6-Ig (Ki-67 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 66555-6-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66555-6-Ig (Ki-67 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 66555-6-Ig

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 66555-6-Ig (Ki-67 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 66555-6-Ig

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 66555-6-Ig (Ki-67 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 66555-6-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Ki-67 antibody (66555-6-Ig, Clone: 1B9H2 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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  IF Staining of HeLa using 66555-6-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Ki-67 antibody (66555-6-Ig, Clone: 1B9H2 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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  FC experiment of A431 using 66555-6-Ig

  1x10^6 A431 cells were intracellularly stained with 0.2 μg Ki-67 Monoclonal antibody (66555-6-Ig, Clone:1B9H2,red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005), Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA.

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Applications testées

• Résultats positifs en IHC: tissu d'amygdalite humain, human ovary cancer tissue, human rectal cancer tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431, cellules HeLa
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:1000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.2 μg per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66555-6-Ig cible Ki-67 dans les applications de IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Ki-67 Protéine recombinante Ag26266
• Nom complet: antigen identified by monoclonal antibody Ki-67
• Masse moléculaire calculée: 359 kDa
• Numéro d’acquisition GenBank: NM_002417
• Symbole du gène: KI67
• Identification du gène (NCBI): 4288
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

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